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Secondary

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Product group Antibodies
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence
  • SizePrice
Product group Antibodies
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence
  • SizePrice
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
  • SizePrice
Western Blot of Mouse anti-Human F(c) antibody. Lane 1: Human IgG (p/n 009-0102). Lane 2: Human Fc (p/n 009-0103).
Lane 3: Human Fab (p/n 009-0105).
Lane 4: Human Fab2 (p/n 009-0104).
Lane 5: Human IgM (p/n 009-0107).
Lane 6: Human IgM Fc5µ (p/n 009-0131). Load: 50 ng per lane.
Primary antibody: Anti-Human F(c) monoclonal antibody at 1:1000 for overnight at 4°C.
Secondary antibody: HRP mouse secondary antibody at 1:40,000 for 30 min at RT.
Block: MB-070 for 30 min at RT.
Predicted MW: ~28kDa.
Observed MW: ~33kDa for Human Fc.
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA
ReactivityHuman
  • SizePrice
Western Blot of Mouse anti-Human IgM Fc5µ antibody.
Lane 1: Human IgM (p/n 009-0107).
Lane 2: Human IgG (p/n 009-0102).
Lane 3: Human Fc (p/n 009-0103).
Lane 4: Human Fab (p/n 009-0105).
Lane 5: Human Fab2 (p/n 009-0104).
Lane 6: Human IgM Fc5µ (p/n 009-0131).
Load: 50 ng whole molecule per lane.
Primary antibody: anti-Human IgM Fc5µ monoclonal antibody at 1:1000 for overnight at 4° C.
Secondary antibody: Peroxidase anti-mouse secondary antibody at 1:40,000 for 30 min at RT.
Block: MB-070 for 30 min at RT.
Predicated/Observed size: 78 kDa, 78 kDa for Human IgM.
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA
ReactivityHuman
  • SizePrice
Rockland primary and DyLight conjugated secondary antibodies were used to detect: Human transferrin (1° 109-4134, green 2° 611-743-127); Alpha 1 anti trypsin (1° 100-101-147, red 2° 605-742-125); and Human IgG (1° 109-3102, Blue 2° 610-741-124) in a multiplex fluorescent western blot of human serum.
Each primary antibody was diluted to 1:1000 in blocking buffer (MB-070) and incubated for 2 hrs at RT. Secondary antibodies diluted 1:10000 in blocking buffer and incubated ~ 1hr at 4°C. Imaged using the Biorad VersaDoc 4000.
Product group Antibodies
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence
  • SizePrice
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
  • SizePrice
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
Product group Antibodies
OriGene
ApplicationsImmunoPrecipitation
  • SizePrice

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