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Product group Antibodies
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence
  • SizePrice
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
Dot Blot of Mouse IgM (mu chain) (GOAT) Antibody. Lane 1A: Mouse IgM (100 ng). Lane 1B: Mouse IgG (100 ng). Lanes 2-5: serial 3-fold dilution. Primary Antibody: Mouse IgM (mu chain) Antibody at 1 microg/mL at RT for 60 minutes. Secondary Antibody: Donkey Anti-Goat IgG Antibody HRP Conjd (p/n 605-703-125) at 1;40,000 dilution of 1 mg/mL at RT for 30 minutes. Block: MB-070 at RT for 30 min.
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
ELISA results of purified Goat anti-Mouse IgG Antibody Min X human serum tested against purified Mouse IgG. Each well was coated in duplicate with 1.0 microg of Mouse IgG [red line], Human IgG [green line]. The starting dilution of antibody was 5microg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer MB-060-1000, Gt-anti-Donkey HRP conjugate 616-1302, and TMB-1000 substrate.
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
ELISA Results of purified Goat Anti-Mouse IgG F(c) Min X Bovine Horse and Human Serums. Each well was coated in duplicate with 1.0 microg of Mouse IgG (010-0102). The working dilution is 27300. The starting dilution of antibody was 5microg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer (p/n MB-076), Dky Anti- Goat IgG HRP conjugated (p/n 605-703-125) and TMB substrate (p/n TMBE-1000).
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
Western Blot of Goat anti-Mouse IgG Fluorescein Conjugated Secondary Antibody. Lane 1: Mouse IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Fluorescein goat secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Mouse IgG. Other band(s): None.
Product group Antibodies
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot
  • SizePrice
Product group Antibodies
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence
  • SizePrice
Product group Antibodies
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence
  • SizePrice
Product group Antibodies
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence
  • SizePrice
Western Blot of Peroxidase conjugated Goat anti-Mouse IgG antibody. Lane 1: Mouse IgG. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Peroxidase goat secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 30 min at RT. Predicted/Observed size: 50 kDa, 25 kDa for Mouse IgG. Other band(s): none.
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
ELISA Results of Goat Anti-Mouse IgG F(ab)2 Antibody Peroxidase Conjugated tested against purified Mouse IgG F(ab)2. Each well was coated in duplicate with 1.0 microg of Mouse IgG (p/n 010-0102) [Red Line], Mouse IgG F(ab)2 (p/n 010-0104) [Green Line]. The working dilution is 1:38,000. The starting dilution of antibody was 5microg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer (p/n MB-076) and TMB substrate (p/n TMBE-1000).
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice

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