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Secondary

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Product group Antibodies
OriGene
Mouse IgG (H&L) Antibody Rhodamine ConjugatedTA397922
ApplicationsFlow Cytometry, ImmunoFluorescence
  • SizePrice
ELISA Results of Donkey Anti-Mouse IgG Antibody tested against purified Mouse IgG. Each well was coated in duplicate with 1.0 microg of Mouse IgG (p/n 010-0102). The working dilution is 1:128,000. The starting dilution of antibody was 5microg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugation Stabilizer (p/n MB-076), Goat Anti-Donkey HRP conjugated (p/n 616-1302) and TMB substrate (p/n TMBE-1000).
Product group Antibodies
OriGene
Mouse IgG (H&L) AntibodyTA397923
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
Western Blot of Fluorescein Donkey Anti-Mouse IgG secondary antibody. Lane 1: Mouse IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Fluorescein donkey secondary antibody at 1:1,000 for 60 min at RT. Blocking: MB-070 for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Mouse IgG. Other band(s): None.
Product group Antibodies
OriGene
Mouse IgG (H&L) Antibody Fluorescein ConjugatedTA397924
ApplicationsFlow Cytometry, ImmunoFluorescence
  • SizePrice
Western Blot of Donkey anti-Mouse IgG (H&L) Antibody Peroxidase Conjugated. Lane 1: Mouse IgG. Lane 2: None. Load: 50 ng per lane. Primary Antibody: None. Secondary antibody: Mouse IgG (H&L) Antibody Peroxidase Conjugated at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 55 and 28 kDa, 55 and 28 kDa for Mouse IgG. Other Band(s): None.
Product group Antibodies
OriGene
Mouse IgG (H&L) Antibody Peroxidase ConjugatedTA397925
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
Product group Antibodies
OriGene
Mouse IgG (H&L) Antibody Alkaline Phosphatase ConjugatedTA397926
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
ELISA results of purified Donkey anti-Mouse IgG Biotin conjugated Antibody tested against Mouse IgG. Each well was coated in duplicate with 1.0 microg of Mouse IgG (purple line). The starting dilution of antibody was 5microg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer MB-060-1000, Streptavidin HRP conjugate, and TMB-1000 substrate.
Product group Antibodies
OriGene
Mouse IgG (H&L) Antibody Biotin ConjugatedTA397927
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
Western Blot of Texas Red™ Donkey Anti-Mouse IgG secondary antibody. Lane 1: Mouse IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Texas Red™ donkey secondary antibody at 1:1,000 for 60 min at RT. Blocking: MB-070 for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Mouse IgG. Other band(s): None.
Product group Antibodies
OriGene
Mouse IgG (H&L) Antibody Texas Red(tm) ConjugatedTA397928
ApplicationsFlow Cytometry, ImmunoFluorescence
  • SizePrice
Western Blot of Goat anti-Rabbit IgG Rhodamine Conjugated Secondary Antibody. Lane 1: Rabbit IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Rhodamine goat secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Rabbit IgG. Other band(s): None.
Product group Antibodies
OriGene
Rabbit IgG (H&L) Antibody Rhodamine ConjugatedTA397929
ApplicationsFlow Cytometry, ImmunoFluorescence
  • SizePrice
Western Blot of Goat anti-Rabbit IgG Pre-Absorbed Phycoerythrin Conjugated Antibody. Lane 1: Rabbit IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Phycoerythrin goat secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 28 & 55 kDa, 55 kDa for Rabbit IgG. Other band(s): None.
Product group Antibodies
OriGene
Rabbit IgG (H&L) Antibody Phycoerythrin conjugated Pre-AdsorbedTA397931
ApplicationsFlow Cytometry, ImmunoFluorescence
  • SizePrice
Western Blot of Anti-Rabbit IgG (H&L) (GOAT) Antibody (p/n 611-1102). Lane M: 3 microl Molecular Ladder. Lane 1: Rabbit IgG whole molecule (p/n 011-0102). Lane 2: Rabbit IgG F(ab) Fragment (p/n 011-0105). Lane 3: Rabbit IgG F(c) Fragment (p/n 010-0103). Lane 4: Rabbit IgM Whole Molecule (p/n 011-0107). Lane 5: Normal Rabbit Serum (p/n B309). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (GOAT) Antibody (p/n 611-1102) 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody (p/n CUST10) 1:40,000 in MB-070 for 30 min at RT. Predicted/Obsevered Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.
Product group Antibodies
OriGene
Rabbit IgG (H&L) Secondary Antibody Fluorescein ConjugatedTA397933
ApplicationsFlow Cytometry, ImmunoFluorescence
  • SizePrice
Western Blot of Peroxidase conjugated Goat anti-Rabbit IgG antibody. Lane 1: Rabbit IgG. Lane 2: none. Load: 25 ng per lane. Primary antibody: none. Secondary antibody: Peroxidase goat secondary antibody at 1:40,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 55 kDa, 28 kDa for Rabbit IgG. Other band(s): none.
Product group Antibodies
OriGene
Rabbit IgG (H&L) Antibody Peroxidase Conjugated Pre-AdsorbedTA397934
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
Western Blot of Peroxidase conjugated Goat anti-Rabbit IgG antibody. Lane 1: Rabbit IgG. Lane 2: none. Load: 25 ng per lane. Primary antibody: none. Secondary antibody: Peroxidase goat secondary antibody at 1:40,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 55 kDa, 28 kDa for Rabbit IgG. Other band(s): none.
Product group Antibodies
OriGene
Rabbit IgG (H&L) Antibody Peroxidase Conjugated Pre-AdsorbedTA397935
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
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