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Product group Antibodies
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence
  • SizePrice
ELISA results of purified Donkey Anti-Sheep IgG peroxidase conjugated MX8 Antibody tested against purified Sheep IgG. Each well was coated in duplicate with 1.0 microg of Sheep IgG (green line). The starting dilution of antibody was 5microg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using TMB-1000 substrate.
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
ELISA results of purified Polyclonal Donkey Anti-Sheep IgG Antibody Alkaline Phosphatase Conjugated tested against Sheep IgG. Each well was coated in duplicate with 1.0microg of antigen. The starting dilution of antibody was 5microg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Buffer (p/n NPP-B500) and Substrate (p/n NPP-10).
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
Product group Antibodies
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence
  • SizePrice
ELISA results of purified ELISA of Donkey anti-Sheep IgG Antibody tested against purified Sheep IgG. Each well was coated in duplicate with 1.0 microg of Sheep IgG. The starting dilution of antibody was 5microg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer MB-060-1000, Gt-a-Donkey IgG HRP conjugate 1:10,000, and TMB-8000 substrate.
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
Dot Blot of Sheep IgG Antibody Fluorescein Conjugated. Antigen: Sheep IgG Load: Lane 1 - 50 ng Lane 2 - 16.67 ng Lane 3 - 5.56 ng Lane 4 - 1.85 ng Lane 5 - 0.62 ng. Primary antibody: none. Secondary antibody: Sheep IgG Antibody Fluorescein Conjugated 1:1,000 for 60 min at RT. Block: MB-070 for 1 HR at RT.
Product group Antibodies
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence
  • SizePrice
ELISA results of purified Donkey anti-Sheep IgG Antibody Peroxidase Conjugated tested against purified Sheep IgG. Each well was coated in duplicate with 1.0 microg of Sheep IgG (p/n 003-0102). The starting dilution of antibody was 5microg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer and TMB substrate p/n TMBE-1000.
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
ELISA results of purified Anti-SHEEP IgG (H&L) (DONKEY) Alkaline Phosphatase Conjugated tested against Sheep IgG. Each well was coated in 1.0 microg of antigen. The starting dilution of antibody was 5 microg/mL and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Buffer (p/n pNPP Buffer) and Substrate (p/n TMB-8000).
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
ELISA results of purified Donkey anti-Sheep IgG Biotin Conjugated Antibody tested against Sheep IgG. Each well was coated in duplicate with 1.0 microg of Sheep IgG. The starting dilution of antibody was 5microg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer MB-060-1000, Streptavidin HRP conjugate, and TMB substrate.
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
Western Blot of Donkey anti-Sheep IgG Texas Red Conjugated Antibody. Lane 1: Sheep IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Texas Red donkey secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 28 & 55 kDa, 28 & 55 kDa for Sheep IgG. Other band(s): None.
Product group Antibodies
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence
  • SizePrice
Product group Antibodies
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence
  • SizePrice

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