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Dot Blot results of Goat Anti-Dog IgG Antibody Fluorescein Conjugate.
Dots are Dog IgG: (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. Primary Antibody: none.
Secondary Antibody: Goat Anti-Dog IgG Antibody FITC at 1ug/mL in MB-070 1hr RT.
Imaged with BioRad ChemiDoc, Fluorescein filter.
Product group Antibodies
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence
  • SizePrice
Product group Antibodies
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence
  • SizePrice
ELISA Results of Purified Goat Anti-Dog IgG Antibody Peroxidase Conjugated tested against purified Dog IgG. Each well was coated in duplicate with 1.0 µg of Dog IgG (p/n 004-0102). The working dilution is 1:36,000. The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Stabilizer (p/n MB-076) and TMB substrate (p/n TMBE-1000).
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
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ELISA Results of Goat Anti-Dog IgG F(c) Antibody Peroxidase Conjugated tested against purified Dog IgG F(c) HRP. Each well was coated in duplicate with 1.0 µg of Dog IgG F(c) (p/n 003-0103). The working dilution is 1:46,500. The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody.
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
ELISA Results of Goat Anti-Dog IgG Antibody Alkaline Phosphatase Conjugated tested against purified Dog IgG Alk Phos. Each well was coated in duplicate with 1.0 µg of Dog IgG (p/n 004-0102). The working dilution is 1:8000. The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Alkaline Phosphatase Substrate ELISA Buffer (p/n NPP-10) and NPP Working Buffer (p/n NPP-B500).
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
ELISA Results of Goat Anti-Dog IgG F(c) Antibody Biotin Conjugated tested against purified Dog IgG F(c). Each well was coated in duplicate with 1.0 µg of Dog IgG F(c) (p/n 004-0103). The working dilution is 1:88,000. The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP stabilizer (p/n MB-076), Streptavidin HRP conjugated (p/n S000-03) and TMB substrate (p/n TMBE-1000).
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
Product group Antibodies
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence
  • SizePrice
Product group Antibodies
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence
  • SizePrice
Product group Antibodies
OriGene
ApplicationsImmunoFluorescence
ReactivityGoat
  • SizePrice
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityGoat
  • SizePrice

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