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ELISA of purified Mouse Anti-Human IgG Antibody Peroxidase Conjugated tested against purified Human IgG HRP. Each well was coated in duplicate with 1.0 µg of Human IgG (p/n 009-0102). The working dilution is 1:28,000. The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using TMB substrate (p/n TMBE-1000).
Product group Antibodies
OriGene
Human IgG (H&L) Antibody Peroxidase ConjugatedTA397776
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
  • SizePrice
ELISA Results of Sheep Anti-Human IgG Antibody tested against purified Human IgG. Each well was coated in duplicate with 1.0 µg of Human IgG (p/n 009-0102). The working dilution is 1:60000. The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugation Stabilizer (p/n MB-076), Donkey Anti-Sheep IgG HRP conjugated (p/n 613-703-168) and TMB substrate (p/n TMBE-1000).
Product group Antibodies
OriGene
Human IgG (H&L) AntibodyTA397779
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
ELISA results of purified Sheep Anti-Human IgG Peroxidase Conjugated Antibody tested against purified Human IgG. Each well was coated in duplicate with 1.0 µg of Human IgG (red line). The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using TMB-1000 substrate.
Product group Antibodies
OriGene
Human IgG (H&L) Antibody Peroxidase ConjugatedTA397781
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
  • SizePrice
Dot Blot of Sheep anti-Human IgG Antibody Alkaline Phosphatase Conjugated. Antigen: Human IgG. Load: Lane 1 - 200 ng Lane 2 - 66.67 ng Lane 3 - 22.22 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng. Primary antibody: none. Secondary antibody: Sheep anti-Human IgG Antibody Alkaline Phosphatase Conjugated at 1:1,000 for 60 min at RT. Block: MB-070 for 60 min at RT. Visualized using NBT-100 Alkaline Phosphatase Substrate for 30 seconds at RT.
Product group Antibodies
OriGene
Human IgG (H&L) Antibody Alkaline Phosphatase ConjugatedTA397782
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
  • SizePrice
ELISA results of purified Sheep Anti-HUMAN IgG Biotin Conjugated Antibody tested against purified Human IgG. Each well was coated in duplicate with 1.0 µg of human IgG (green line). The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer MB-060-1000, Streptavidin HRP conjugate 1:10,000 and TMB-1000 substrate.
Product group Antibodies
OriGene
Human IgG (H&L) Antibody Biotin ConjugatedTA397783
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
ELISA Results of Purified Donkey Anti-Human IgG Peroxidase Conjugated Antibody tested against purified Human IgG protein. Each well was coated in duplicate with 10 µg of Human IgG (p/n 009-0102). The working dilution of Anti-Human IgG HRP is 1:30,000. The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS Blocking buffer (p/n MB-066) and TMBE-1000 substrate.
Product group Antibodies
OriGene
Human IgG (H&L) Antibody Peroxidase ConjugatedTA397791
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
ELISA results of Purified Donkey Anti-Human IgG MinX10 Antibody Alkaline Phosphatase Conjugated tested against purified Human IgG mx10. Each well was coated in duplicate with 10µg of Human IgG (p/n 009-0102). The working dilution of Anti-Human IgG mx10 Alkaline Phosphatase Antibody is 1:34,000. The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel Blocking buffer (p/n MB-066) and NPP Substrate (p/n NPP-100).
Product group Antibodies
OriGene
Human IgG (H&L) Antibody Alkaline Phosphatase Conjugated Pre-AdsorbedTA397794
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
ELISA Results of Goat Anti-Mouse IgG2b (Gamma 2b chain) Antibody Minimum Cross Reactivity to Bovine, Human, and Rabbit Serum Proteins tested against purified Mouse IgG2b mx3. Each well was coated in duplicate with 1.0 µg of Mouse IgG2b (p/n 010-0142). The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer (p/n MB-076), Donkey Anti-Goat IgG HRP conjugated (p/n 605-703-125), and TMB substrate (p/n TMBE-1000).
Product group Antibodies
OriGene
Mouse IgG2b Antibody Pre-adsorbedTA397812
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityMouse
  • SizePrice
Dot Blot showing the detection of Mouse IgG. A three-fold serial dilution of Mouse IgG starting at 200ng was spotted onto 0.45 ?m nitrocellulose. After blocking in 5% Blotto (p/n B501-0500) 1 Hour at 20°C, Anti-Mouse IgG (H&L) (GOAT) Antibody Fluorescien Conjugated (p/n 610-102-121) secondary antibody was used at 1:1000 in Blocking Buffer for Fluorescent Western Blotting (p/n MB-070) and imaged using the Bio-Rad VersaDoc® 4000 MP.
Product group Antibodies
OriGene
Mouse IgG (H&L) Secondary Antibody Fluorescein Conjugated Pre-AdsorbedTA397821
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityMouse
  • SizePrice
ELISA results of purified Goat anti-Mouse IgG2a Antibody Peroxidase conjugated tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 1.0 µg of Mouse IgG2a. The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer and TMB substrate p/n TMBE-1000.
Product group Antibodies
OriGene
Mouse IgG2a Antibody Peroxidase Conjugated Pre-adsorbedTA397823
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityMouse
  • SizePrice
ELISA results of purified Goat Anti-MOUSE IgG IgA IgM Peroxidase Antibody (Min X Bv, Hs, Hu Serum Proteins) tested against purified MOUSE IgG IgA IgM. Each well was coated in duplicate with 1.0 µg of MOUSE IgG (red line) IgA (green line) IgM (blue line). The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using TMB-1000 substrate.
Product group Antibodies
OriGene
Mouse IgG IgA IgM (H&L) Antibody Peroxidase Conjugated Pre-AdsorbedTA397827
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityMouse
  • SizePrice
Dot Blot of Goat anti-Mouse IgG antibody Alkaline Phosphatase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Rb Rt & Sh Serum Proteins. Antigen: Mouse IgG. Load: 200 ng, 66.7 ng, 22.2 ng, 7.41 ng, or 2.47 ng as indicated. Primary antibody: Anti-Mouse IgG antibody Alkaline Phosphatase Conjugated at 1:1,000 for 60 min at 4°C. Secondary antibody: N/A. Block: Blocking Buffer for Fluorescent Western Blotting (MB-070) for 60 min at RT.
Product group Antibodies
OriGene
Mouse IgG (H&L) Secondary Antibody Alkaline Phosphatase Conjugated Pre-AdsorbedTA397831
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityMouse
  • SizePrice
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