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SEMA3B / SemA Antibody

LS-C409102
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSEMA3B
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SEMA3B / SemA Antibody
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IHC (1:50 - 1:200), WB (1:500 - 1:2000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID7869
  • Target name
    SEMA3B
  • Target description
    semaphorin 3B
  • Target synonyms
    LUCA-1, SEMA5, SEMAA, SemA, semaV, semaphorin-3B, sema A(V), sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3B, semaphorin A, semaphorin-V
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SEMA3B using anti-SEMA3B antibody (A06559-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates Lane 2: human U2OS whole cell lysates Lane 3: human A549 whole cell lysates Lane 4: human U-87MG whole cell lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SEMA3B antigen affinity purified polyclonal antibody (Catalog # A06559-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SEMA3B at approximately 83KD. The expected band size for SEMA3B is at 83KD.
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