Paraformaldehyde-fixed, paraffin embedded Mouse cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with SEMA6D Polyclonal Antibody, Unconjugated (bs-2943R) at 1:400 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with SEMA6D Polyclonal Antibody, Unconjugated (bs-2943R) at 1:400 overnight at 4°C, DAB staining.
SEMA6D Polyclonal Antibody
BS-2943R
ApplicationsImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Equine, Human, Mouse, Porcine, Rat
TargetSEMA6D
Overview
- SupplierBioss
- Product NameSEMA6D Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID80031
- Target nameSEMA6D
- Target descriptionsemaphorin 6D
- Target synonymssemaphorin-6D, sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D
- HostRabbit
- IsotypeIgG
- ReactivityBovine, Canine, Equine, Human, Mouse, Porcine, Rat
- Storage Instruction-20°C
- UNSPSC41116161
Datasheet
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