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SENP1 Polyclonal Antibody

RD76686A
Reddot Biotech
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetSENP1
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Overview

  • Supplier
    Reddot Biotech
  • Product Name
    SENP1 Polyclonal Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.6 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID29843
  • Target name
    SENP1
  • Target description
    SUMO specific peptidase 1
  • Target synonyms
    SuPr-2, sentrin-specific protease 1, SUMO1/sentrin specific peptidase 1, SUMO1/sentrin specific protease 1, sentrin/SUMO-specific protease SENP1
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    This gene encodes a cysteine protease that specifically targets members of the small ubiquitin-like modifier (SUMO) protein family. This protease regulates SUMO pathways by deconjugating sumoylated proteins. This protease also functions to process the precursor SUMO proteins into their mature form. Alternate splicing results in multiple transcript variants.
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SENP1 using anti-SENP1 antibody (A02156-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human CACO-2 whole cell lysates, Lane 2: human HEPG2 whole cell lysates, Lane 3: human HELA whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: human U87 whole cell lysates, Lane 6: human Raji whole cell lysates, Lane 7: human HL-60 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SENP1 antigen affinity purified polyclonal antibody (Catalog # A02156-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SENP1 at approximately 73KD. The expected band size for SENP1 is at 73KD.
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