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Western Blot analysis of COLO205 cells using Septin 1 Polyclonal Antibody
Western Blot analysis of COLO205 cells using Septin 1 Polyclonal Antibody
Western Blot analysis of COLO205 cells using Septin 1 Polyclonal Antibody

SEPT1 Antibody

CSB-PA004067
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSEPTIN1
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Overview

  • Supplier
    Cusabio
  • Product Name
    SEPT1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1731
  • Target name
    SEPTIN1
  • Target description
    septin 1
  • Target synonyms
    DIFF6; differentiation 6 (deoxyguanosine triphosphate triphosphohydrolase); LARP; peanut-like protein 3; PNUTL3; SEP1; SEPT1; septin-1; serologically defined breast cancer antigen NY-BR-24
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8WYJ6
  • Protein Name
    Septin-1
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of LARP/SEPTIN1 using anti-LARP/SEPTIN1 antibody (A30537-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human MOLT-4 whole cell lysates, Lane 3: human Daudi whole cell lysates, Lane 4: rat thymus tissue lysates, Lane 5: mouse thymus tissue lysates, Lane 6: mouse EL-4 whole cell lysates. red to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LARP/SEPTIN1 antigen affinity purified polyclonal antibody (Catalog # A30537-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LARP/SEPTIN1 at approximately 40 kDa. The expected band size for LARP/SEPTIN1 is at 40 kDa.
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