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45181 Polyclonal Antibody

CAC10482
Biomatik
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
ReactivityMouse
TargetSEPTIN12
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Overview

  • Supplier
    Biomatik
  • Product Name
    45181 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Applications Supplier
    ELISA, WB, IF; Recommended dilution: WB:1:500-1:5000, IF:1:50-1:200
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID124404
  • Target name
    SEPTIN12
  • Target description
    septin 12
  • Target synonyms
    SEPT12, SPGF10, septin-12, testicular tissue protein Li 168
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8IYM1
  • Protein Name
    Septin-12
  • Scientific Description
    The 45181 Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, WB, IF.
  • Reactivity
    Mouse
  • Reactivity Supplier
    Human, Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SEPTIN12 using anti-SEPTIN12 antibody (A12342-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat testis tissue lysates, Lane 3: mouse brain tissue lysates, Lane 4: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SEPTIN12 antigen affinity purified polyclonal antibody (Catalog # A12342-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SEPTIN12 at approximately 45 kDa. The expected band size for SEPTIN12 is at 41 kDa.
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