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Western Blot analysis of 293 cells using Septin 3 Polyclonal Antibody
Western Blot analysis of 293 cells using Septin 3 Polyclonal Antibody
Western Blot analysis of 293 cells using Septin 3 Polyclonal Antibody

SEPT3 Antibody

CSB-PA004068
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSEPTIN3
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Overview

  • Supplier
    Cusabio
  • Product Name
    SEPT3 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID55964
  • Target name
    SEPTIN3
  • Target description
    septin 3
  • Target synonyms
    SEP3, SEPT3, bK250D10.3, neuronal-specific septin-3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UH03
  • Protein Name
    Neuronal-specific septin-3
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Septin 3/SEPTIN3 using anti-Septin 3/SEPTIN3 antibody (A30742-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Daudi whole cell lysates, Lane 2: human SiHa whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates. red to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Septin 3/SEPTIN3 antigen affinity purified polyclonal antibody (Catalog # A30742-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Septin 3/SEPTIN3 at approximately 41 kDa. The expected band size for Septin 3/SEPTIN3 is at 41 kDa.
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