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ProductsBack/Septin 2 antibody [18S62]
WB analysis of Jurkat whole cell lysate using GTX04847 Septin 2 antibody [18S62].
WB analysis of Jurkat whole cell lysate using GTX04847 Septin 2 antibody [18S62].
WB analysis of Jurkat whole cell lysate using GTX04847 Septin 2 antibody [18S62].

Septin 2 antibody [18S62]

GTX04847
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetSEPTIN2
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Overview

  • Supplier
    GeneTex
  • Product Name
    Septin 2 antibody [18S62]
  • Delivery Days Customer
    7
  • Application Supplier Note
    WB: 1:500-1:2000. ICC/IF: 1:50-1:200. IHC-P: 1:50-1:200. FACS: 1:50. IP: 1:50. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    18S62
  • Conjugate
    Unconjugated
  • Gene ID4735
  • Target name
    SEPTIN2
  • Target description
    septin 2
  • Target synonyms
    DIFF6, NEDD-5, NEDD5, Pnutl3, SEPT2, Septin-2, hNedd5, septin-2, epididymis secretory sperm binding protein, neural precursor cell expressed, developmentally down-regulated 5
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Septin 2/SEPTIN2 using anti-Septin 2/SEPTIN2 antibody (A34007). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: monkey COS-7 whole cell lysates, Lane 6: human HepG2 whole cell lysates, Lane 7: human HEL whole cell lysates. red to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Septin 2/SEPTIN2 antigen affinity purified polyclonal antibody (Catalog # A34007) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Septin 2/SEPTIN2 at approximately 41 kDa. The expected band size for Septin 2/SEPTIN2 is at 41 kDa.
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