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SERCA2 ATPase Antibody (YA1931, Rabbit)

HY-P82186
MedChem Express
TargetATP2A2
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    SERCA2 ATPase Antibody (YA1931, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID488
  • Target name
    ATP2A2
  • Target description
    ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 2
  • Target synonyms
    ATP2B, DAR, DD, RHABDO2, SERCA2, sarcoplasmic/endoplasmic reticulum calcium ATPase 2, ATPase Ca++ transporting cardiac muscle slow twitch 2, ATPase, Ca++ dependent, slow-twitch, cardiac muscle-2, SR Ca(2+)-ATPase 2, calcium pump 2, cardiac Ca2+ ATPase, endoplasmic reticulum class 1/2 Ca(2+) ATPase
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    SERCA2 ATPase Antibody (YA1931) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA1931), targeting SERCA2 ATPase, with a predicted molecular weight of 115 kDa (observed band size: 115 kDa). SERCA2 ATPase Antibody (YA1931) can be used for WB, IHC-F, IHC-P, ICC/IF experiment in human, rat background.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ATP2A2 using anti-ATP2A2 antibody (RP1054). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATP2A2 antigen affinity purified polyclonal antibody (Catalog # RP1054) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATP2A2 at approximately 115 kDa. The expected band size for ATP2A2 is at 115 kDa.
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