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ProductsBack/Serine racemase (SRR) Mouse Monoclonal Antibody [Clone ID: OTI1D7]
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SRR (RC210359, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SRR. Positive lysates LY402897 (100ug) and LC402897 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SRR (RC210359, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SRR. Positive lysates LY402897 (100ug) and LC402897 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SRR (RC210359, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SRR. Positive lysates LY402897 (100ug) and LC402897 (20ug) can be purchased separately from OriGene.

Serine racemase (SRR) Mouse Monoclonal Antibody [Clone ID: OTI1D7]

CF500942
OriGene
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSRR
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    Serine racemase (SRR) Mouse Monoclonal Antibody [Clone ID: OTI1D7]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI1D7
  • Gene ID63826
  • Target name
    SRR
  • Target description
    serine racemase
  • Target synonyms
    ILV1, ISO1, serine racemase, D-serine ammonia-lyase, D-serine dehydratase, L-serine ammonia-lyase, L-serine dehydratase
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDQ9GZT4
  • Protein Name
    Serine racemase
  • Scientific Description
    Carrier-free (BSA/glycerol-free) SRR mouse monoclonal antibody, clone OTI1D7 (formerly 1D7)
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of Serine racemase/SRR using anti-Serine racemase/SRR antibody (A02660-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: monkey COS-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Serine racemase/SRR antigen affinity purified polyclonal antibody (Catalog # A02660-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Serine racemase/SRR at approximately 37 kDa. The expected band size for Serine racemase/SRR is at 37 kDa.
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