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SERPINA12 antibody [N2C3]

GTX117695
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetSERPINA12
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Overview

  • Supplier
    GeneTex
  • Product Name
    SERPINA12 antibody [N2C3]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:1000-1:10000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.57 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID145264
  • Target name
    SERPINA12
  • Target description
    serpin family A member 12
  • Target synonyms
    OL-64; serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 12; serpin A12; serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 12; vaspin; visceral adipose tissue-derived serine protease inhibitor; visceral adipose-specific SERPIN
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8IW75
  • Protein Name
    Serpin A12
  • Scientific Description
    May modulates insulin action conceivably only in the presence of its yet undefined target proteases in white adipose tissues.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Plasma levels and placental expression of vaspin in pregnant women with diabetes mellitus. Huo Y et al., 2015 Mar, Braz J Med Biol Res
    Read more

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Figure 1. Western blot analysis of Vaspin/SERPINA12 using anti-Vaspin/SERPINA12 antibody (A05352-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse B16-F10 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Vaspin/SERPINA12 antigen affinity purified polyclonal antibody (Catalog # A05352-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Vaspin/SERPINA12 at approximately 55 kDa. The expected band size for Vaspin/SERPINA12 is at 47 kDa.
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