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Western Blot analysis of 4T1 cells using AACT Polyclonal Antibody.
Western Blot analysis of 4T1 cells using AACT Polyclonal Antibody.
Western Blot analysis of 4T1 cells using AACT Polyclonal Antibody.

SERPINA3 Antibody

CSB-PA005627
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetSERPINA3
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Overview

  • Supplier
    Cusabio
  • Product Name
    SERPINA3 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID12
  • Target name
    SERPINA3
  • Target description
    serpin family A member 3
  • Target synonyms
    AACT; ACT; alpha-1-antichymotrypsin; cell growth-inhibiting gene 24/25 protein; GIG24; GIG25; growth-inhibiting protein 24; growth-inhibiting protein 25; serine (or cysteine) proteinase inhibitor, clade A, member 3; serpin A3; serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP01011
  • Protein Name
    Alpha-1-antichymotrypsin
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of AACT/SERPINA3 using anti-AACT/SERPINA3 antibody (A02312-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human hepatocellular carcinoma tumor tissue (HCCT) lysates, Lane 3: human HUH-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AACT/SERPINA3 antigen affinity purified polyclonal antibody (Catalog # A02312-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AACT/SERPINA3 at approximately 50 kDa. The expected band size for AACT/SERPINA3 is at 48 kDa.
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