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Western Blot analysis of Hela cells using PAI-3 Polyclonal Antibody
Western Blot analysis of Hela cells using PAI-3 Polyclonal Antibody
Western Blot analysis of Hela cells using PAI-3 Polyclonal Antibody

SERPINA5 Antibody

CSB-PA003700
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSERPINA5
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Overview

  • Supplier
    Cusabio
  • Product Name
    SERPINA5 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5104
  • Target name
    SERPINA5
  • Target description
    serpin family A member 5
  • Target synonyms
    PAI-3, PAI3, PCI, PCI-B, PLANH3, PROCI, plasma serine protease inhibitor, acrosomal serine protease inhibitor, plasminogen activator inhibitor III, plasminogen activator inhibitor-3, protein C inhibitor, serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 5, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP05154
  • Protein Name
    Plasma serine protease inhibitor
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SERPINA5 using anti-SERPINA5 antibody (A01916-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: human SKOV3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SERPINA5 antigen affinity purified polyclonal antibody (Catalog # A01916-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SERPINA5 at approximately 46 kDa. The expected band size for SERPINA5 is at 46KD.
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