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Western blot All lanes: SERPINB8 antibody at 2microg/ml + Jurkat whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 43, 28, 23 kDa Observed band size: 43 kDa
Western blot All lanes: SERPINB8 antibody at 2microg/ml + Jurkat whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 43, 28, 23 kDa Observed band size: 43 kDa
Western blot All lanes: SERPINB8 antibody at 2microg/ml + Jurkat whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 43, 28, 23 kDa Observed band size: 43 kDa

SERPINB8 Antibody

CSB-PA021076EA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSERPINB8
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Overview

  • Supplier
    Cusabio
  • Product Name
    SERPINB8 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5271
  • Target name
    SERPINB8
  • Target description
    serpin family B member 8
  • Target synonyms
    C18orf53; CAP2; cytoplasmic antiproteinase 2; peptidase inhibitor 8; PI8; PI-8; protease inhibitor 8 (ovalbumin type); PSS5; serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 8; serpin B8; serpin peptidase inhibitor, clade B (ovalbumin), member 8
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP50452
  • Protein Name
    Serpin B8
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SERPINB8 using anti-SERPINB8 antibody (A11265-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SERPINB8 antigen affinity purified polyclonal antibody (Catalog # A11265-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SERPINB8 at approximately 48 kDa. The expected band size for SERPINB8 is at 43 kDa.
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