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Immunohistochemistry of paraffin-embedded Human liver cancer tissue using SERPINF2 Polyclonal Antibody at dilution 1:50
Immunohistochemistry of paraffin-embedded Human liver cancer tissue using SERPINF2 Polyclonal Antibody at dilution 1:50
Immunohistochemistry of paraffin-embedded Human liver cancer tissue using SERPINF2 Polyclonal Antibody at dilution 1:50

SERPINF2 Polyclonal Antibody

E-AB-16155
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    SERPINF2 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications Supplier
    ELISA IHC
  • Certification
    Research Use Only
  • Concentration
    0.6mg/ml
  • Scientific Description
    This gene encodes a member of the serpin family of serine protease inhibitors. The protein is a major inhibitor of plasmin, which degrades fibrin and various other proteins. Consequently, the proper function of this gene has a major role in regulating the blood clotting pathway. Mutations in this gene result in alpha-2-plasmin inhibitor deficiency, which is characterized by severe hemorrhagic diathesis. Multiple transcript variants encoding different isoforms have been found for this gene.
  • UNSPSC
    12352203

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Lane 1: Liver lysates probed with Alpha 2 antiplasmin Polyclonal Antibody, Unconjugated (bs-6188R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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Figure 1. Western blot analysis of Alpha 2 Antiplasmin/SERPINF2 using anti-Alpha 2 Antiplasmin/SERPINF2 antibody (A04248-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human HCCT tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Alpha 2 Antiplasmin/SERPINF2 antigen affinity purified polyclonal antibody (Catalog # A04248-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Alpha 2 Antiplasmin/SERPINF2 at approximately 65 kDa. The expected band size for Alpha 2 Antiplasmin/SERPINF2 is at 65 kDa.
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