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ProductsBack/SERPINH1 / HSP47 Antibody (aa222-251)

SERPINH1 / HSP47 Antibody (aa222-251)

LS-C168975
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetSERPINH1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SERPINH1 / HSP47 Antibody (aa222-251)
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC, IHC-P (1:50 - 1:100), WB (1:100 - 1:200)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID871
  • Target name
    SERPINH1
  • Target description
    serpin family H member 1
  • Target synonyms
    47 kDa heat shock protein; arsenic-transactivated protein 3; AsTP3; CBP1; CBP2; cell proliferation-inducing gene 14 protein; collagen binding protein 1; colligin-1; colligin-2; gp46; HSP47; OI10; PIG14; PPROM; RA-A47; rheumatoid arthritis antigen A-47; rheumatoid arthritis-related antigen RA-A47; serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1); serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 2, (collagen-binding protein 2); serpin H1; serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1); SERPINH2
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Hsp47/SERPINH1 using anti-Hsp47/SERPINH1 antibody (PB9325). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human SK-N-SH whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: rat lung tissue lysates, Lane 5: rat kidney tissue lysates, Lane 6: rat L6 whole cell lysates, Lane 7: mouse lung tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hsp47/SERPINH1 antigen affinity purified polyclonal antibody (Catalog # PB9325) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Hsp47/SERPINH1 at approximately 46 kDa. The expected band size for Hsp47/SERPINH1 is at 46 kDa.
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