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ProductsBack/Seryl-tRNA synthetase antibody [N1C2]
Immunoprecipitation of Seryl-tRNA synthetase protein from 293T whole cell extracts using 5 μg of Seryl-tRNA synthetase antibody [N1C2] (GTX113546). Western blot analysis was performed using Seryl-tRNA synthetase antibody [N1C2] (GTX113546). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Immunoprecipitation of Seryl-tRNA synthetase protein from 293T whole cell extracts using 5 μg of Seryl-tRNA synthetase antibody [N1C2] (GTX113546). Western blot analysis was performed using Seryl-tRNA synthetase antibody [N1C2] (GTX113546). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Immunoprecipitation of Seryl-tRNA synthetase protein from 293T whole cell extracts using 5 μg of Seryl-tRNA synthetase antibody [N1C2] (GTX113546). Western blot analysis was performed using Seryl-tRNA synthetase antibody [N1C2] (GTX113546). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Seryl-tRNA synthetase antibody [N1C2]

GTX113546
GeneTex
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSARS1
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Overview

  • Supplier
    GeneTex
  • Product Name
    Seryl-tRNA synthetase antibody [N1C2]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.51 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6301
  • Target name
    SARS1
  • Target description
    seryl-tRNA synthetase 1
  • Target synonyms
    NEDMAS, SARS, SERRS, SERS, serine--tRNA ligase, cytoplasmic, serine tRNA ligase 1, cytoplasmic, seryl-tRNA synthetase, cytoplasmic, seryl-tRNA(Ser/Sec) synthetase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP49591
  • Protein Name
    Serine--tRNA ligase, cytoplasmic
  • Scientific Description
    This gene belongs to the class II amino-acyl tRNA family. The encoded enzyme catalyzes the transfer of L-serine to tRNA (Ser) and is related to bacterial and yeast counterparts. [provided by RefSeq]
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Seryl-TRNA Synthetase/SERS/SARS1 using anti-Seryl-TRNA Synthetase/SERS/SARS1 antibody (A00501-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Seryl-TRNA Synthetase/SERS/SARS1 antigen affinity purified polyclonal antibody (Catalog # A00501-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Seryl-TRNA Synthetase/SERS/SARS1 at approximately 59 kDa. The expected band size for Seryl-TRNA Synthetase/SERS/SARS1 is at 59 kDa.
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