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SETD1A antibody

GTX130194
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetSETD1A
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Overview

  • Supplier
    GeneTex
  • Product Name
    SETD1A antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.18 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID9739
  • Target name
    SETD1A
  • Target description
    SET domain containing 1A, histone lysine methyltransferase
  • Target synonyms
    EPEDD, EPEO2, KMT2F, NEDSID, Set1, Set1A, histone-lysine N-methyltransferase SETD1A, SET domain-containing protein 1A, hSET1A, lysine N-methyltransferase 2F, set1/Ash2 histone methyltransferase complex subunit SET1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO15047
  • Protein Name
    Histone-lysine N-methyltransferase SETD1A
  • Scientific Description
    SET1A is a component of a histone methyltransferase (HMT) complex that produces mono-, di-, and trimethylated histone H3 at Lys4. The complex is the analog of the S. cerevisiae Set1/COMPASS complex (Lee and Skalnik, 2005 [PubMed 16253997]). Also see SET1B (MIM 611055).[supplied by OMIM]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Imuta H, Fujita D, Oba S, et al. Histone methylation and demethylation are implicated in the transient and sustained activation of the interleukin-1β gene in murine macrophages. Heart Vessels. 2020,35(12):1746-1754. doi: 10.1007/s00380-020-01670-5
    Read this paper

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Figure 1. Western blot analysis of SET1/SETD1A using anti-SET1/SETD1A antibody (A03736-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human SW620 whole cell lysates, Lane 2: human U87 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SET1/SETD1A antigen affinity purified polyclonal antibody (Catalog # A03736-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SET1/SETD1A at approximately 186KD. The expected band size for SET1/SETD1A is at 186KD.
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