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Western Blot analysis of 293 cells using Splicing factor 1 Polyclonal Antibody
Western Blot analysis of 293 cells using Splicing factor 1 Polyclonal Antibody
Western Blot analysis of 293 cells using Splicing factor 1 Polyclonal Antibody

SF1 Antibody

CSB-PA004139
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetSF1
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Overview

  • Supplier
    Cusabio
  • Product Name
    SF1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7536
  • Target name
    SF1
  • Target description
    splicing factor 1
  • Target synonyms
    BBP; D11S636; mammalian branch point-binding protein; MBBP; splicing factor 1; transcription factor ZFM1; ZCCHC25; ZFM1; zinc finger gene in MEN1 locus; zinc finger protein 162; ZNF162
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15637
  • Protein Name
    Splicing factor 1
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of splicing factor 1 using anti-splicing factor 1 antibody (A01009). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SK-OV-3 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: mouse NIH/3T3 whole cell lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-splicing factor 1 antigen affinity purified polyclonal antibody (Catalog # A01009) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for splicing factor 1 at approximately 68-80 kDa. The expected band size for splicing factor 1 is at 68 kDa.
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