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ProductsBack/SF3A2 / SF3a66 Antibody

SF3A2 / SF3a66 Antibody

LS-C497518
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSF3A2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SF3A2 / SF3a66 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 49kDa, while the observed MW by Western blot was 66kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) The predicted MW is 49kDa, while the observed MW by Western blot was 66kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID8175
  • Target name
    SF3A2
  • Target description
    splicing factor 3a subunit 2
  • Target synonyms
    PRP11, PRPF11, SAP62, SF3a66, splicing factor 3A subunit 2, SAP 62, pre-mRNA splicing factor SF3A, subunit 2, spliceosome associated protein 62, splicing factor 3a, subunit 2, 66kD, splicing factor 3a, subunit 2, 66kDa
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SF3A2 using anti-SF3A2 antibody (A11289-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates, Lane 5: rat thymus tissue lysates, Lane 6: rat ovary tissue lysates, Lane 7: mouse thymus tissue lysates, Lane 8: mouse ovary tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SF3A2 antigen affinity purified polyclonal antibody (Catalog # A11289-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SF3A2 at approximately 66 kDa. The expected band size for SF3A2 is at 49,60,66 kDa.
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