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Western Blot analysis of K562 cells using SAP 49 Polyclonal Antibody
Western Blot analysis of K562 cells using SAP 49 Polyclonal Antibody
Western Blot analysis of K562 cells using SAP 49 Polyclonal Antibody

SF3B4 Antibody

CSB-PA006438
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSF3B4
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Overview

  • Supplier
    Cusabio
  • Product Name
    SF3B4 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10262
  • Target name
    SF3B4
  • Target description
    splicing factor 3b subunit 4
  • Target synonyms
    AFD1, Hsh49, SAP49, SF3b49, splicing factor 3B subunit 4, SAP 49, SF3b50, pre-mRNA-splicing factor SF3b 49 kDa subunit, spliceosomal protein, spliceosome-associated protein (U2 snRNP), spliceosome-associated protein 49, splicing factor 3b, subunit 4, 49kD, splicing factor 3b, subunit 4, 49kDa
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15427
  • Protein Name
    Splicing factor 3B subunit 4
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SF3B4 using anti-SF3B4 antibody (A06414-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human U2OS whole cell lysates, Lane 3: human HGC-27 whole cell lysates, Lane 4: human GES-1 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat stomach tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse stomach tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SF3B4 antigen affinity purified polyclonal antibody (Catalog # A06414-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SF3B4 at approximately 44 kDa. The expected band size for SF3B4 is at 44 kDa.
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