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ProductsBack/SFRP2 antibody [C2C3], C-term

SFRP2 antibody [C2C3], C-term

GTX111892
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetSFRP2
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Overview

  • Supplier
    GeneTex
  • Product Name
    SFRP2 antibody [C2C3], C-term
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.12 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6423
  • Target name
    SFRP2
  • Target description
    secreted frizzled related protein 2
  • Target synonyms
    FRP-2, SARP1, SDF-5, secreted frizzled-related protein 2, SARP-1, sFRP-2, secreted apoptosis related protein 1, testicular tissue protein Li 170
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96HF1
  • Protein Name
    Secreted frizzled-related protein 2
  • Scientific Description
    This gene encodes a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. Methylation of this gene is a potential marker for the presence of colorectal cancer. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Langerhans cells and SFRP2/Wnt/beta-catenin signalling control adaptation of skin epidermis to mechanical stretching. Ledwon JK et al., 2022 Feb, J Cell Mol Med
    Read more
  • The Role of Wnt/beta-Catenin Pathway Mediators in Aortic Valve Stenosis. Khan K et al., 2020, Front Cell Dev Biol
    Read more

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Figure 1. Western blot analysis of SFRP2 using anti-SFRP2 antibody (A01752-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human U-87MG whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SFRP2 antigen affinity purified polyclonal antibody (Catalog # A01752-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SFRP2 at approximately 38KD. The expected band size for SFRP2 is at 33KD.
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