Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using FRP 2(GTX111892) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
![SFRP2 antibody [C2C3], C-term detects SFRP2 protein by immunofluorescent analysis. Sample: HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SFRP2 protein stained by SFRP2 antibody [C2C3], C-term (GTX111892) diluted at 1:500. Blue: Hoechst 33342 staining.](https://www.genetex.com/upload/website/prouct_img/normal/GTX111892/GTX111892_42718_20170628_IFA_w_23060500_411.webp "SFRP2 antibody [C2C3], C-term detects SFRP2 protein by immunofluorescent analysis. Sample: HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SFRP2 protein stained by SFRP2 antibody [C2C3], C-term (GTX111892) diluted at 1:500. Blue: Hoechst 33342 staining.")
![Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SFRP2 antibody [C2C3], C-term (GTX111892) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](https://www.genetex.com/upload/website/prouct_img/normal/GTX111892/GTX111892_44608_20221014_WB_TPM_watermark_25042920_256.webp "Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SFRP2 antibody [C2C3], C-term (GTX111892) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using FRP 2(GTX111892) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
SFRP2 antibody [C2C3], C-term
GTX111892
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Porcine
TargetSFRP2
Overview
- SupplierGeneTex
- Product NameSFRP2 antibody [C2C3], C-term
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1.34 mg/ml
- ConjugateUnconjugated
- Gene ID6423
- Target nameSFRP2
- Target descriptionsecreted frizzled related protein 2
- Target synonymsFRP-2, SARP1, SDF-5, secreted frizzled-related protein 2, SARP-1, sFRP-2, secreted apoptosis related protein 1, testicular tissue protein Li 170
- HostRabbit
- IsotypeIgG
- Protein IDQ96HF1
- Protein NameSecreted frizzled-related protein 2
- Scientific DescriptionThis gene encodes a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. Methylation of this gene is a potential marker for the presence of colorectal cancer. [provided by RefSeq]
- ReactivityHuman, Porcine
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
Datasheet
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