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SFRP4 Monoclonal Antibody

CAB4189
Assay Genie
TargetSFRP4
Product group Antibodies
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Overview

  • Supplier
    Assay Genie
  • Product Name
    SFRP4 Rabbit mAb
  • Delivery Days Customer
    9
  • Applications Supplier
    WB,IHC,IF
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID6424
  • Target name
    SFRP4
  • Target description
    secreted frizzled related protein 4
  • Target synonyms
    frizzled protein, human endometrium; FRP-4; FRPHE; FRZB-2; PYL; secreted frizzled-related protein 4; secreted frizzled-related protein 4; secreted frizzled-related protein 4; sFRP-4
  • Host
    Rabbit
  • Protein IDQ6FHJ7
  • Protein Name
    Secreted frizzled-related protein 4
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity Supplier
    Human,Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SFRP4 using anti-SFRP4 antibody (PB9888). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: A549 whole cell lysates, Lane 2: SW620 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SFRP4 antigen affinity purified polyclonal antibody (Catalog # PB9888) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SFRP4 at approximately 49 kDa. The expected band size for SFRP4 is at 40 kDa.
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