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SFTPB / Surfactant Protein B Antibody

LS-C408347
LifeSpan BioSciences
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetSFTPB
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SFTPB / Surfactant Protein B Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 42kDa, while the observed MW by Western blot was 50kDa.
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IHC (1:50 - 1:200), IP (1:20 - 1:50), WB (1:500 - 1:2000) The predicted MW is 42kDa, while the observed MW by Western blot was 50kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    2.82 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID6439
  • Target name
    SFTPB
  • Target description
    surfactant protein B
  • Target synonyms
    18 kDa pulmonary-surfactant protein; 6 kDa protein; PSP-B; pulmonary surfactant-associated protein B; pulmonary surfactant-associated proteolipid SPL(Phe); SFTB3; SFTP3; SMDP1; SP-B
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SFTPB using anti-SFTPB antibody (A03441-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human COLO-320 whole cell lysates, Lane 3: human HepG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SFTPB antigen affinity purified polyclonal antibody (Catalog # A03441-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SFTPB at approximately 46KD. The expected band size for SFTPB is at 42KD.
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