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Immunohistochemistry of paraffin-embedded human colon tissue using CSB-PA846614LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human colon tissue using CSB-PA846614LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human colon tissue using CSB-PA846614LA01HU at dilution of 1:100

SGTB Antibody

CSB-PA846614LA01HU
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSGTB
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Overview

  • Supplier
    Cusabio
  • Product Name
    SGTB Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID54557
  • Target name
    SGTB
  • Target description
    small glutamine rich tetratricopeptide repeat co-chaperone beta
  • Target synonyms
    beta-SGT; SGT2; small glutamine rich protein with tetratricopeptide repeats 2; small glutamine rich tetratricopeptide repeat containing beta; small glutamine-rich tetratricopeptide repeat (TPR)-containing, beta; small glutamine-rich tetratricopeptide repeat-containing protein beta
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96EQ0
  • Protein Name
    Small glutamine-rich tetratricopeptide repeat-containing protein beta
  • Scientific Description
    Co-chaperone that binds directly to HSC70 and HSP70 and regulates their ATPase activity.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SGT2/SGTB using anti-SGT2/SGTB antibody (A13056-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: rat brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SGT2/SGTB antigen affinity purified polyclonal antibody (Catalog # A13056-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SGT2/SGTB at approximately 36 kDa. The expected band size for SGT2/SGTB is at 34 kDa.
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