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SH2D3C / NSP3 Antibody

LS-C779941
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetSH2D3C
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SH2D3C / NSP3 Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA (1:5000 - 1:20000), WB (1:500 - 1:2000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID10044
  • Target name
    SH2D3C
  • Target description
    SH2 domain containing 3C
  • Target synonyms
    CHAT, NSP3, PRO34088, SHEP1, SH2 domain-containing protein 3C, SH2 domain-containing Eph receptor-binding protein 1, cas/HEF1-associated signal transducer, chat-H, novel SH2-containing protein 3
  • Host
    Rabbit
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SH2D3C using anti-SH2D3C antibody (A04497-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SH2D3C antigen affinity purified polyclonal antibody (Catalog # A04497-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SH2D3C at approximately 94 kDa. The expected band size for SH2D3C is at 94 kDa.
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