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SH2D4A Antibody (Internal)

LS-C386042
LifeSpan BioSciences
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetSH2D4A
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SH2D4A Antibody (Internal)
  • Delivery Days Customer
    23
  • Applications
    ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA (1:20000), IHC (1:100 - 1:300)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID63898
  • Target name
    SH2D4A
  • Target description
    SH2 domain containing 4A
  • Target synonyms
    PPP1R38, SH2A, SH2 domain-containing protein 4A, protein SH(2)A, protein phosphatase 1 regulatory subunit 38
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SH2D4A using anti-SH2D4A antibody (A09094-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: rat PC-12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SH2D4A antigen affinity purified polyclonal antibody (Catalog # A09094-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SH2D4A at approximately 50 kDa. The expected band size for SH2D4A is at 53 kDa.
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