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SH3GL3 antibody

GTX114758
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetSH3GL3
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Overview

  • Supplier
    GeneTex
  • Product Name
    SH3GL3 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:10000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6457
  • Target name
    SH3GL3
  • Target description
    SH3 domain containing GRB2 like 3, endophilin A3
  • Target synonyms
    CNSA3, EEN-B2, HsT19371, SH3D2C, SH3P13, endophilin-A3, SH3 domain containing GRB2 like endophilin A3, SH3 domain protein 2C, SH3 domain-containing GRB2-like protein 3, SH3-domain GRB2-like 3, endophilin-3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ99963
  • Protein Name
    Endophilin-A3
  • Scientific Description
    Implicated in endocytosis. May recruit other proteins to membranes with high curvature.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SH3GL3 using anti-SH3GL3 antibody (A09836-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SH3GL3 antigen affinity purified polyclonal antibody (Catalog # A09836-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SH3GL3 at approximately 42 kDa. The expected band size for SH3GL3 is at 39 kDa.
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