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SH3KBP1 / CIN85 Antibody

LS-C402025
LifeSpan BioSciences
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetSH3KBP1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SH3KBP1 / CIN85 Antibody
  • Delivery Days Customer
    23
  • Applications
    ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, IHC (1:30 - 1:150)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.6 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID30011
  • Target name
    SH3KBP1
  • Target description
    SH3 domain containing kinase binding protein 1
  • Target synonyms
    AGMX2, CD2BP3, CIN85, GIG10, HSB-1, HSB1, IMD61, MIG18, SH3 domain-containing kinase-binding protein 1, CD2-binding protein 3, SH3-domain kinase binding protein 1, Src family kinase-binding protein 1, c-Cbl-interacting protein, cbl-interacting protein of 85 kDa, human Src family kinase-binding protein 1, migration-inducing gene 18, src-related kinase binding protein-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SH3KBP1 using anti-SH3KBP1 antibody (A02835-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human Raji whole cell lysates, Lane 4: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SH3KBP1 antigen affinity purified polyclonal antibody (Catalog # A02835-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SH3KBP1 at approximately 85 kDa. The expected band size for SH3KBP1 is at 85 kDa.
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