Immunofluorescence staining of Hela cells with CSB-PA773800LA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
SH3RF1 Antibody
CSB-PA773800LA01HU
ApplicationsImmunoFluorescence, ELISA
Product group Antibodies
ReactivityHuman
TargetSH3RF1
Overview
- SupplierCusabio
- Product NameSH3RF1 Antibody
- Delivery Days Customer20
- ApplicationsImmunoFluorescence, ELISA
- CertificationResearch Use Only
- ClonalityPolyclonal
- ConjugateUnconjugated
- Gene ID57630
- Target nameSH3RF1
- Target descriptionSH3 domain containing ring finger 1
- Target synonymsE3 ubiquitin-protein ligase SH3RF1; plenty of SH3 domains; plenty of SH3s; POSH; putative E3 ubiquitin-protein ligase SH3RF1; ring finger protein 142; RING-type E3 ubiquitin transferase SH3RF1; RNF142; SH3 domain-containing RING finger protein 1; SH3 multiple domains 2; SH3 multiple domains protein 2; SH3MD2
- HostRabbit
- IsotypeIgG
- Protein IDQ7Z6J0
- Protein NameE3 ubiquitin-protein ligase SH3RF1
- Scientific DescriptionActs as a scaffold protein, contributes to Rac-induced signal transduction such as JNKs (MAPK8 and MAPK9) activation and induces apoptosis. Within a signaling complex, it probably recruits protein kinases such as MAP3K10 or MAP3K11 which are in turn activated leading to the sequential activation of MAP2K4, MAP2K7 and JNKs (MAPK8 and MAPK9) (By similarity). May be involved in targeting of HIV-1 GAG and GAG-POL polyproteins to the plasma membrane.
- ReactivityHuman
- Storage Instruction-20°C or -80°C
- UNSPSC12352203