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SHANK3 Antibody (aa1475-1525)

LS-C290822
LifeSpan BioSciences
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
ReactivityHuman, Mouse
TargetSHANK3
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SHANK3 Antibody (aa1475-1525)
  • Delivery Days Customer
    23
  • Applications
    ImmunoPrecipitation, Western Blot
  • Applications Supplier
    IP (2 - 10 µg/mg lysate), WB (1:2000 - 1:10000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID85358
  • Target name
    SHANK3
  • Target description
    SH3 and multiple ankyrin repeat domains 3
  • Target synonyms
    DEL22q13.3, PROSAP2, PSAP2, SCZD15, SPANK-2, SH3 and multiple ankyrin repeat domains protein 3, proline rich synapse associated protein 2, shank postsynaptic density protein, shank3 postsynaptic density protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SHANK3 using anti-SHANK3 antibody (A01231-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SHANK3 antigen affinity purified polyclonal antibody (Catalog # A01231-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SHANK3 at approximately 180-190KD. The expected band size for SHANK3 is at 185KD.
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