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Western blot All lanes: SHC4 antibody at 6ug/ml + Jurkat whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 69, 43 kDa Observed band size: 69 kDa
Western blot All lanes: SHC4 antibody at 6ug/ml + Jurkat whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 69, 43 kDa Observed band size: 69 kDa
Western blot All lanes: SHC4 antibody at 6ug/ml + Jurkat whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 69, 43 kDa Observed band size: 69 kDa

SHC4 Antibody

CSB-PA747568ESR1HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSHC4
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Overview

  • Supplier
    Cusabio
  • Product Name
    SHC4 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID399694
  • Target name
    SHC4
  • Target description
    SHC adaptor protein 4
  • Target synonyms
    RaLP, SHCD, SHC-transforming protein 4, SH2 domain protein C4, SHC (Src homology 2 domain containing) family, member 4, SHC-transforming protein D, hShcD, rai-like protein, src homology 2 domain-containing-transforming protein C4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ6S5L8
  • Protein Name
    SHC-transforming protein 4
  • Scientific Description
    Activates both Ras-dependent and Ras-independent migratory pathways in melanomas. Contributes to the early phases of agrin-induced tyrosine phosphorylation of CHRNB1.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SHCD/SHC4 using anti-SHCD/SHC4 antibody (A10841-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SHCD/SHC4 antigen affinity purified polyclonal antibody (Catalog # A10841-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SHCD/SHC4 at approximately 69 kDa. The expected band size for SHCD/SHC4 is at 69 kDa.
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