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SHCBP1L / C1orf14 Antibody (aa525-574)

LS-C145929
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetSHCBP1L
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SHCBP1L / C1orf14 Antibody (aa525-574)
  • Delivery Days Customer
    14
  • Applications
    Western Blot
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID81626
  • Target name
    SHCBP1L
  • Target description
    SHC binding and spindle associated 1 like
  • Target synonyms
    C1orf14, GE36, testicular spindle-associated protein SHCBP1L, SHC SH2 domain-binding protein 1-like protein, SHC SH2-domain binding protein 1-like
  • Host
    Rabbit
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SHCBP1L using anti-SHCBP1L antibody (A15056-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: rat testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SHCBP1L antigen affinity purified polyclonal antibody (Catalog # A15056-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SHCBP1L at approximately 90 kDa. The expected band size for SHCBP1L is at 73 kDa.
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