Sheep anti Human IgG4 (subclass specific)

Nordic-MUbio

Sheep anti Human IgG4 (subclass specific)

7

Precipitating polyclonal Sheep antisera to Human IgG4, subclass specific.

Precipitation assays. Because of their characteristics and those of the subclass-specific antigenic determinants, the use of this antiserum requires special precautions. The specificity and reactivity depend largely on the conditions of the test system in which they are applied. The performance of NORDIC polyclonal subclass-specific antisera is guaranteed when they are used for the purposes described in RWP 5E/88, applying the special immunoprecipitation arrangement recommended for these purposes. In immunoelectrophoresis use 2 microl 1:8, 1:16 and 1:32 dilutions of pathological serum or equivalent against 60 microl antiserum. In double radial immunodiffusion use a rosette arrangement with 10 microl antiserum in 3 mm diameter center well and 2 microl serum samples (neat and serially diluted) in 2 mm diameter peripheral wells. Nordic also makes available a human standard serum with assigned values of the subclasses of IgG (NOR-01). The antigenic differences between subclasses are considerably less pronounced than those between classes or between light chain types. Subclass-specific determinants are only few in number and probably restricted in their structural exposure, which makes them relative weak immunogens. This explains why the titres of subclass-specific antisera are usually lower than those of class-specific antisera which react with many antigenic determinants commonly present on all molecules of the respective class.

Secondary antibodies

Research Use Only

Polyclonal

Sheep

Purified homogenous IgG4 is isolated from human serum. Freunds complete adjuvant is used in the first step of the immunization procedure.

12352203