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ProductsBack/Sheep anti Human Protein Phosphatase 4 R1 (X/A)

Sheep anti Human Protein Phosphatase 4 R1 (X/A)

P210P
Nordic-MUbio
ApplicationsWestern Blot
Product group Antibodies
TargetPPP4R1
100 ug
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Overview

  • Supplier
    Nordic-MUbio
  • Product Name
    Sheep anti Human Protein Phosphatase 4 R1 (X/A)
  • Delivery Days Customer
    7
  • Application Supplier Note
    Detects protein phosphatase X/A (PP4 R1) protein by Western blot (5-10 microg/ml). Detects a 125 kDa band in bovine testes lysate. Optimal concentration should be evaluated by serial dilutions.
  • Applications
    Western Blot
  • Applications Supplier
    Western Blotting;Western Blotting
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9989
  • Target name
    PPP4R1
  • Target description
    protein phosphatase 4 regulatory subunit 1
  • Target synonyms
    MEG1, PP4(Rmeg), PP4R1, serine/threonine-protein phosphatase 4 regulatory subunit 1, serine/threonine phosphatase 4
  • Host
    Sheep
  • Isotype
    IgG
  • Protein IDQ8TF05
  • Protein Name
    Serine/threonine-protein phosphatase 4 regulatory subunit 1
  • Scientific Description
    Serine/Threonine-Protein Phosphatase 4 Regulatory Subunit 1; Protein Phosphatase X/A'2; PP4R1
  • Shelf life instruction
    See expiration date on vial
  • Reactivity Supplier
    Human;Mouse;Rat
  • Reactivity Supplier Note
    Antibody raised against synthetic peptide corresponding to amino acids 38 to 52 of the human protein phosphatase 4R1.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PPP4R1 using anti-PPP4R1 antibody (A14144-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: monkey COS-7 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat stomach tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse stomach tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPP4R1 antigen affinity purified polyclonal antibody (Catalog # A14144-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPP4R1 at approximately 130 kDa. The expected band size for PPP4R1 is at 130 kDa.
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