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ProductsBack/Sheep anti Human secretory component (free and bound)

Sheep anti Human secretory component (free and bound)

ShAHu/SC/7S
Nordic-MUbio
ApplicationsDot Blot, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPIGR
10 mg
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Overview

  • Supplier
    Nordic-MUbio
  • Product Name
    Sheep anti Human secretory component (free and bound)
  • Delivery Days Customer
    7
  • Application Supplier Note
    Indirect immunofluorescence,ELISA,Dot blot,Immunoblotting.
  • Applications
    Dot Blot, Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Immunoblotting;Dot blot;ELISA;Immunohistochemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID5284
  • Target name
    PIGR
  • Target description
    polymeric immunoglobulin receptor
  • Target synonyms
    polymeric immunoglobulin receptor, hepatocellular carcinoma associated protein TB6, poly-Ig receptor
  • Host
    Sheep
  • Protein IDP01833
  • Protein Name
    Polymeric immunoglobulin receptor
  • Source
    Secretory component is present in human secretions bound to secretory IgA (sIgA) and in free form. Secretory IgA (sIgA) functions as a dimer or polymer and accounts for almost all specific mucosal antibody activity. A molecule of sIgA is made up of ...
  • Reactivity
    Human
  • Reactivity Supplier
    Human
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PIGR using anti-PIGR antibody (PB9776). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions. Lane 1: HELA Whole Cell Lysate, Lane 2: SKOV Whole Cell Lysate, Lane 3: MCF-7 Whole Cell Lysate, Lane 4: SW620 Whole Cell Lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIGR antigen affinity purified polyclonal antibody (Catalog # PB9776) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PIGR at approximately 83 kDa. The expected band size for PIGR is at 83 kDa.
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