Sheep anti Rat Serum proteins

Nordic-MUbio

Sheep anti Rat Serum proteins

7

Precipitating polyclonal Sheep antiSerum to Rat Serum proteins.

Precipitation assays. In immunoelectrophoresis use 2 microl serum or equivalent against 120 microl antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 microl antiserum in 3 mm diameter center well and 2 microl serum samples (neat and serially diluted in 2 mm diameter peripheral wells. Different bleedings of the immunized animals are pooled to obtain a broad spectrum balanced against the varying concentrations of the individual serum protein components.

Secondary antibodies

Research Use Only

Polyclonal

Sheep

Pooled whole rat serum and partly purified serum fractions. Freunds complete adjuvant is used in the first step of the immunization procedure.

-20°C,2°C to 8°C

12352203