Sheep SELP(Selectin, Platelet) ELISA Kit

Biorbyt

Sheep SELP(Selectin, Platelet) ELISA Kit

10

standard: 400 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sheep SELP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sheep SELP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sheep SELP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Sheep SELP in the samples is then determined by comparing the OD of the samples to the standard curve

6.25-400 ng/mL

2.62 ng/mL

Research Use Only

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Selectin, Platelet(SELP). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Selectin, Platelet(SELP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Selectin, Platelet(SELP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Selectin, Platelet(SELP) in the samples is then determined by comparing the OD of the samples to the standard curve.

41116158