WB analysis of HEK293T cells transfected with Shh plasmid (Right) or empty vector (Left) for 48 hrs using GTX83651 Shh antibody [10H6]. Loading : 5 ug per lane
![WB analysis of various cell lines using GTX83651 Shh antibody [10H6]. Loading : 35 ug per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX83651/GTX83651_3792_WB_w_23061420_710.webp "WB analysis of various cell lines using GTX83651 Shh antibody [10H6]. Loading : 35 ug per lane")
![ICC/IF analysis of COS7 cells transiently transfected with Shh plasmid using GTX83651 Shh antibody [10H6].](https://www.genetex.com/upload/website/prouct_img/normal/GTX83651/GTX83651_760_ICCIF_w_23061420_555.webp "ICC/IF analysis of COS7 cells transiently transfected with Shh plasmid using GTX83651 Shh antibody [10H6].")
WB analysis of HEK293T cells transfected with Shh plasmid (Right) or empty vector (Left) for 48 hrs using GTX83651 Shh antibody [10H6]. Loading : 5 ug per lane
Shh antibody [10H6]
GTX83651
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityCanine, Human, Monkey, Rat
TargetSHH
Overview
- SupplierGeneTex
- Product NameShh antibody [10H6] - Overexpression Validated
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000-1:2000. ICC/IF: 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID10H6
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID6469
- Target nameSHH
- Target descriptionsonic hedgehog signaling molecule
- Target synonymsHHG1; HLP3; HPE3; MCOPCB5; shh unprocessed N-terminal signaling and C-terminal autoprocessing domains; ShhNC; SMMCI; sonic hedgehog homolog; sonic hedgehog protein; TPT; TPTPS
- HostMouse
- IsotypeIgG1
- Protein IDQ15465
- Protein NameSonic hedgehog protein
- Scientific DescriptionBinds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. In the absence of SHH, PTC represses the constitutive signaling activity of SMO. Also regulates another target, the gli oncogene. Intercellular signal essential for a variety of patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Displays both floor plate- and motor neuron-inducing activity. The threshold concentration of N-product required for motor neuron induction is 5-fold lower than that required for floor plate induction.
- ReactivityCanine, Human, Monkey, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203