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ProductsBack/SHIP (INPP5D) Rabbit Polyclonal Antibody
Gel: 6%SDS-PAGE Lysate: 40 microg Lane: Ramos cell lysate Primary antibody: TA365014 (INPP5D Antibody) at dilution 1/800 Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution Exposure time: 15 seconds
Gel: 6%SDS-PAGE Lysate: 40 microg Lane: Ramos cell lysate Primary antibody: TA365014 (INPP5D Antibody) at dilution 1/800 Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution Exposure time: 15 seconds
Gel: 6%SDS-PAGE Lysate: 40 microg Lane: Ramos cell lysate Primary antibody: TA365014 (INPP5D Antibody) at dilution 1/800 Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution Exposure time: 15 seconds

SHIP (INPP5D) Rabbit Polyclonal Antibody

TA365014
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadMSDS
Product group Antibodies
TargetINPP5D
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    SHIP (INPP5D) Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID3635
  • Target name
    INPP5D
  • Target description
    inositol polyphosphate-5-phosphatase D
  • Target synonyms
    hp51CN; inositol polyphosphate-5-phosphatase, 145kD; inositol polyphosphate-5-phosphatase, 145kDa; p150Ship; phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1; phosphatidylinositol 4,5-bisphosphate 5-phosphatase; SH2 domain-containing inositol 5'-phosphatase 1; SHIP; SHIP1; SHIP-1; signaling inositol polyphosphate 5 phosphatase SIP-145; signaling inositol polyphosphate phosphatase SHIP II; SIP-145
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ92835
  • Protein Name
    Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1
  • Scientific Description
    INPP5D rabbit polyclonal antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Immunohistochemistry analysis in human tonsil and skeletal muscle tissues using HPA070455 antibody. Corresponding INPP5D RNA-seq data are presented for the same tissues.
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Figure 1. Western blot analysis of INPP5D using anti-INPP5D antibody (A03358-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-INPP5D antigen affinity purified polyclonal antibody (Catalog # A03358-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for INPP5D at approximately 145 kDa. The expected band size for INPP5D is at 133 kDa.
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