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SHISA7 Antibody (aa308-337, Biotin)

LS-C258316
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetSHISA7
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SHISA7 Antibody (aa308-337, Biotin)
  • Delivery Days Customer
    23
  • Application Supplier Note
    The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA, WB The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Biotin
  • Estimated Purity
    ...
  • Gene ID729956
  • Target name
    SHISA7
  • Target description
    shisa family member 7
  • Target synonyms
    CKAMP59; cystine-knot AMPAR modulating protein of 59 kDa; GABA(A) receptor auxiliary subunit Shisa7; protein shisa-7; shisa homolog 7; UPF0626 protein A
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SHISA7 using anti-SHISA7 antibody (A18878-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SHISA7 antigen affinity purified polyclonal antibody (Catalog # A18878-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SHISA7 at approximately 70KD. The expected band size for SHISA7 is at 70KD.
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