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SHP1 Antibody (YA1044)

HY-P81307
MedChem Express
TargetPTPN6
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    SHP1 Antibody (YA1044)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID5777
  • Target name
    PTPN6
  • Target description
    protein tyrosine phosphatase non-receptor type 6
  • Target synonyms
    HCP; HCPH; hematopoietic cell phosphatase; hematopoietic cell protein-tyrosine phosphatase; HPTP1C; protein-tyrosine phosphatase 1C; protein-tyrosine phosphatase SHP-1; PTP-1C; SHP1; SHP-1; SHP-1L; SH-PTP1; tyrosine-protein phosphatase non-receptor type 6
  • Host
    Mouse
  • Isotype
    IgG2a
  • Scientific Description
    SHP1 Antibody (YA1044) is a mouse-derived non-conjugated IgG2a antibody (Clone NO.: YA1044), targeting SHP1, with a predicted molecular weight of 68 kDa (observed band size: 68 kDa). SHP1 Antibody (YA1044) can be used for WB, IP experiment in human background.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SHP1/PTPN6 using anti-SHP1/PTPN6 antibody (A00938-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: mouse Raw264.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SHP1/PTPN6 antigen affinity purified polyclonal antibody (Catalog # A00938-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SHP1/PTPN6 at approximately 68KD. The expected band size for SHP1/PTPN6 is at 68KD.
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