
Detection enhancement of Rac1 with treatment of Signal + in Western blot. Treatment with GTX49999 resulted in clear signal, whereas dilution with conventional PBS-T resulted in no signal.
Primary Ab: Anti-Rac1 (1: 1,000)
Secondary Ab: Anti-Mouse IgG-HRP (1: 2,500)
Sample: Rat cortical primary neuron
Detection: ECL
SignalPlus Antibody Enhancer
GTX49999
ApplicationsDot Blot, Western Blot, ELISA
Product group Labware / Accesories
CategoryTechnique Specific Products
Overview
- SupplierGeneTex
- Product NameSignalPlus Antibody Enhancer
- Delivery Days Customer9
- Application Supplier NoteDilute primary antibody in Solution 1 and secondary antibody in Solution 2. We recommend using a 10-fold lower working concentration of primary antibody than is usually required for western blot. Optimal working dilutions should be determined experimentally by the end user.
- ApplicationsDot Blot, Western Blot, ELISA
- CertificationResearch Use Only
- Storage Instruction2°C to 8°C
- UNSPSC41121816
References
- Anti-inflammatory activities of garlic sprouts, a source of alpha-linolenic acid and 5-hydroxy-l-tryptophan, in RAW 264.7 cells. Gdula-Argasinska J et al., 2017, Acta Biochim PolRead this paper
- n-3 Fatty acids as resolvents of inflammation in the A549 cells. Gdula-Argasinska J et al., 2015 Jun, Pharmacol RepRead this paper


