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ProductsBack/SIM2 Antibody

SIM2 Antibody

LS-C750070
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetSIM2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SIM2 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 63kDa/73kDa, while the observed MW by Western blot was 56kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) The predicted MW is 63kDa/73kDa, while the observed MW by Western blot was 56kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID6493
  • Target name
    SIM2
  • Target description
    SIM bHLH transcription factor 2
  • Target synonyms
    HMC13F06, HMC29C01, SIM, bHLHe15, single-minded homolog 2, class E basic helix-loop-helix protein 15, single-minded family bHLH transcription factor 2, transcription factor SIM2
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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IHC image of CSB-PA621763LA01HU diluted at 1:400 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Figure 1. Western blot analysis of SIM2 using anti-SIM2 antibody (A04436-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: monkey COS-7 whole cell lysates, Lane 4: rat skeletal muscle tissue lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse skeletal muscle tissue lysates, Lane 7: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SIM2 antigen affinity purified polyclonal antibody (Catalog # A04436-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SIM2 at approximately 65 kDa. The expected band size for SIM2 is at 73 kDa.
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