Paraformaldehyde-fixed, paraffin embedded human cervical cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-SIPA1L2 Polyclonal Antibody, Unconjugated (bs-7927R) at 1:500 overnight at 4°C, followed by a conjugated secondary for 90 minutes and DAB staining.